Flow Cytometry / Fluorescence Flow Cytometry

Flow cytometry is a technology used for rapid cell count analysis and forms the pillar for a basic blood count test. This is basic technology supporting any pathological laboratory involved in providing blood count services. However, in recent times, along with enumerating basic blood cell count, technological advancements have introduced the possibility to include wide variety of rare cell population enumerations as well, which end up increasing the diagnostic value of the report generated.
Principle
Analyzers on Floor
Instrument Images

PRINCIPLE OF FLOW CYTOMETRY / FLUORESCENCE FLOW CYTOMETRY

Flow cytometry involves enumeration of cells in a liquid flow. The cells are either tagged with fluorescent antibodies or are stained and suspended in a liquid stream within a flow cell. The pressure of the sheath buffer ensures the cells flow in a narrow stream toward the laser source, and the beam hits only one single cell at a time. The incident light on the cell is then scattered forward as well as sideways. This scattered light is captured by the optics in the analyzer and directed to the detector. The cells are analyzed on the basis of their shape, size as well as internal complexity.
For HIV detection, the blood cells are generally tagged using fluorescent labeled antibodies specific for CD3, CD4 and CD8 enumeration. In case of complete blood count analysis, the blood cells are stained differentially and analyzed.
Flowcytometry image