PRINCIPLE OF PHOTOMETRY
The basic principle of this technology involves measurement of quantity of light absorbing analyte in a solution. This can only be applied to solutions which follow the Beer Lambert’s law. Analytes which have the tendency to absorb light, when exposed to a beam of incident light, will absorb some. This results in reflection of a light of lower intensity. The intensity of the reflected light is then considered inversely proportional to the concentration of the analyte of interest in the solution.
The main advantages involve ease of operation and wide variety of parameters which can be covered by this assay.